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yqeL [2020-11-02 13:46:39]
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yqeL [2020-11-02 13:46:39]

ribosomal silencing factor
locus
BSU_25620
pI
4.37
mw
13.16 kDa
protein length
118 aa Sequence Blast
gene length
357 bp Sequence Blast
function
translation
product
ribosomal silencing factor
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]

    • Gene

    Coordinates
    2,642,841 → 2,643,197

    The protein

    Catalyzed reaction/ biological activity

  • silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining [Pubmed|22829778]

    • Protein family

  • Iojap/RsfS family (single member, according to UniProt)

    • Structure

  • [PDB|2O5A] (from B. halodurans, 65% identity)

    • [SW|Interactions]

  • [protein|2BE91EEB70A1961D3BBE0603648E64E86CD0B708|RplN]-[protein|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|RsfS] [Pubmed|22829778], the interaction serves to silence [SW|translation] [Pubmed|22829778]

    • Additional information

  • the protein is enriched in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|RbgA] [Pubmed|23700310]

    • Expression and Regulation

    Operons

    genes
    [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C500 (yqeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2498 NBRP B. subtilis, Japan]
  • BKE25620 (Δ[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCATTCACAAATTCCTCC, downstream forward: _UP4_GATCTTGACTTTGGAATGAA
  • BKK25620 (Δ[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCATTCACAAATTCCTCC, downstream forward: _UP4_GATCTTGACTTTGGAATGAA

    • References

  • 22829778,23700310,22383849